abstract：:Oocytes are usually the largest cells in the body and as such offer unique opportunities for single-cell analysis. Unfortunately, these cells are also some of the rarest in the mammalian female, usually necessitating single-cell analysis. In cases of infertility in humans, determining the quality of the oocyte is ofte...

journal_title：Molecular human reproduction

authors： Brayboy LM,Wessel GM

更新日期：2016-03-01 00:00:00

abstract：:In recent years, advances in imaging probes, cutting-edge microscopy techniques and powerful bioinformatics image analysis have markedly expanded the imaging toolbox available to developmental biologists. Apart from traditional qualitative studies, embryonic development can now be investigated in vivo with improved sp...

journal_title：Molecular human reproduction

authors： Welling M,Ponti A,Pantazis P

更新日期：2016-03-01 00:00:00

abstract：STUDY HYPOTHESIS:In women with preterm premature rupture of the membranes (PPROM), increased oxidative stress may accelerate premature cellular senescence, senescence-associated inflammation and proteolysis, which may predispose them to rupture. STUDY FINDING:We demonstrate mechanistic differences between preterm birt...

journal_title：Molecular human reproduction

authors： Dutta EH,Behnia F,Boldogh I,Saade GR,Taylor BD,Kacerovský M,Menon R

更新日期：2016-02-01 00:00:00

abstract：:Artificial oocyte activation to overcome failed fertilization after intracytoplasmic sperm injection (ICSI) in human oocytes typically employs Ca(2+) ionophores to produce a single cytosolic Ca(2+) increase. In contrast, recombinant phospholipase Czeta (PLCζ) causes Ca(2+) oscillations indistinguishable from those occ...

journal_title：Molecular human reproduction

authors： Sanusi R,Yu Y,Nomikos M,Lai FA,Swann K

更新日期：2015-10-01 00:00:00

abstract：:To mimic post-ovulatory ageing, we have extended the in vitro maturation (IVM) phase to 48 h and examined effects on (i) developmental potential, (ii) expression of a panel of developmentally important genes and (iii) gene-specific epigenetic marks. Results were compared with the 24 h IVM protocol (control) usually em...

journal_title：Molecular human reproduction

authors： Heinzmann J,Mattern F,Aldag P,Bernal-Ulloa SM,Schneider T,Haaf T,Niemann H

更新日期：2015-10-01 00:00:00

abstract：:Uterine contractions are tightly regulated by the electrical activity of myometrial smooth muscle cells (MSMCs). These cells require a depolarizing current to initiate Ca(2+) influx and induce contraction. Cationic leak channels, which permit a steady flow of cations into a cell, are known to cause membrane depolariza...

journal_title：Molecular human reproduction

authors： Reinl EL,Cabeza R,Gregory IA,Cahill AG,England SK

更新日期：2015-10-01 00:00:00

abstract：:Preeclampsia (PE) is a severe pregnancy-induced disorder characterized by hypertension and proteinuria and a leading cause of perinatal maternal-fetal mortality and morbidity in developing countries. Dysregulated human leukocyte antigen (HLA)-G was found in placentas as well as in maternal sera from PE patients; howev...

journal_title：Molecular human reproduction

authors： Tang Y,Liu H,Li H,Peng T,Gu W,Li X

更新日期：2015-09-01 00:00:00

abstract：:Intense macrophage infiltration is observed during luteolysis in various animals including women; however, we still do not know how macrophage infiltration into the human corpus luteum (CL) during luteolysis is regulated. In this study, we examined the expression, localization and regulation of an important chemokine ...

journal_title：Molecular human reproduction

authors： Nio-Kobayashi J,Kudo M,Sakuragi N,Kimura S,Iwanaga T,Duncan WC

更新日期：2015-08-01 00:00:00

abstract：:Prostaglandin F2α (PGF2α) plays a critical role in the initiation and process of parturition. Since human labor has been described as an inflammatory event, we investigated the role of PGF2α in the inflammatory process using cultured human uterine smooth muscle cells (HUSMCs) isolated from term pregnant women as a mod...

journal_title：Molecular human reproduction

authors： Xu C,Liu W,You X,Leimert K,Popowycz K,Fang X,Wood SL,Slater DM,Sun Q,Gu H,Olson DM,Ni X

更新日期：2015-07-01 00:00:00

abstract：:Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa. Interaction between spermatozoa and oviductal epithelial cells improves the fertilizing ability of and reduces chromatin damage in spermatozoa....

journal_title：Molecular human reproduction

authors： Huang VW,Lee CL,Lee YL,Lam KK,Ko JK,Yeung WS,Ho PC,Chiu PC

更新日期：2015-06-01 00:00:00

abstract：:Oocyte vitrification has been introduced into clinical settings without extensive pre-clinical safety testing. In this study, we analysed major safety aspects of human oocyte vitrification in a high security closed system: (i) chromosomal meiotic segregation, (ii) embryonic developmental kinetics and (iii) DNA (hydrox...

journal_title：Molecular human reproduction

authors： De Munck N,Petrussa L,Verheyen G,Staessen C,Vandeskelde Y,Sterckx J,Bocken G,Jacobs K,Stoop D,De Rycke M,Van de Velde H

更新日期：2015-06-01 00:00:00

abstract：:In mammals, egg activation is initiated by multiple cytosolic Ca(2+) transients (Ca(2+) oscillations) that are triggered following delivery of a putative sperm factor from the fertilizing sperm. The identity of this 'sperm factor' thus holds much significance, not only as a vital component in creating a new life, but ...

journal_title：Molecular human reproduction

authors： Kashir J,Nomikos M,Swann K,Lai FA

更新日期：2015-05-01 00:00:00

abstract：:We recently identified the DPY19L2 gene as the main genetic cause of human globozoospermia. Non-genetically characterized cases of globozoospermia were associated with DNA alterations, suggesting that DPY19L2-dependent globozoospermia may be associated with poor DNA quality. However the origins of such defects have no...

journal_title：Molecular human reproduction

authors： Yassine S,Escoffier J,Martinez G,Coutton C,Karaouzène T,Zouari R,Ravanat JL,Metzler-Guillemain C,Lee HC,Fissore R,Hennebicq S,Ray PF,Arnoult C

更新日期：2015-02-01 00:00:00

abstract：:PKDREJ is a testis-specific protein thought to be located on the sperm surface. Functional studies in the mouse revealed that loss of PKDREJ has effects on sperm transport and the ability to undergo an induced acrosome reaction. Thus, PKDREJ has been considered a potential target of post-copulatory sexual selection in...

journal_title：Molecular human reproduction

authors： Vicens A,Gómez Montoto L,Couso-Ferrer F,Sutton KA,Roldan ER

更新日期：2015-02-01 00:00:00

abstract：:Several laboratories have independently isolated mitotically active germ cells, termed female germline stem cells or oogonial stem cells (OSCs), from adult mouse ovaries. However, a recent study using Ddx4-Cre;Rosa26 reporter mice concluded that such germ cells do not exist. Given the disparity in conclusions drawn in...

journal_title：Molecular human reproduction

authors： Park ES,Tilly JL

更新日期：2015-01-01 00:00:00

abstract：:Sperm experience intense and varied selection that dramatically impacts the evolution of sperm quality. Selection acts to ensure that sperm are fertilization-competent and able to overcome the many challenges experienced on their way towards eggs. However, simply being able to fertilize an egg is not enough to ensure ...

journal_title：Molecular human reproduction

authors： Fitzpatrick JL,Lüpold S

更新日期：2014-12-01 00:00:00

abstract：:The epithelium lining the epididymis in the male reproductive tract maintains a luminal environment that promotes sperm cell maturation. This process is dependent on the coordinated expression of many genes that encode proteins with a role in epithelial transport. We previously generated genome-wide maps of open chrom...

journal_title：Molecular human reproduction

authors： Browne JA,Yang R,Song L,Crawford GE,Leir SH,Harris A

更新日期：2014-12-01 00:00:00

abstract：:It is well known that human embryonic stem cells (hESCs) frequently acquire recurrent chromosomal abnormalities, very reminiscent of those found in cancerous cells. Given the parallels between cancer and stem cell biology, we set out to investigate the occurrence of a common form of genome instability in tumors, namel...

journal_title：Molecular human reproduction

authors： Nguyen HT,Markouli C,Geens M,Barbé L,Sermon K,Spits C

更新日期：2014-10-01 00:00:00

abstract：:Cystic fibrosis (CF) is usually considered a rare disease in the Indian population. Two studies have reported on the frequency of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in Indian males with congenital absence of the vas deferens (CAVD), however, data on the spectrum of CFTR gene muta...

journal_title：Molecular human reproduction

authors： Sharma H,Mavuduru RS,Singh SK,Prasad R

更新日期：2014-09-01 00:00:00

abstract：:Retrograde flow of menstrual blood cells during menstruation is considered as the dominant theory for the development of endometriosis. Moreover, current evidence suggests that endometrial-derived stem cells are key players in the pathogenesis of endometriosis. In particular, endometrial stromal stem cells have been s...

journal_title：Molecular human reproduction

authors： Nikoo S,Ebtekar M,Jeddi-Tehrani M,Shervin A,Bozorgmehr M,Vafaei S,Kazemnejad S,Zarnani AH

更新日期：2014-09-01 00:00:00

abstract：:We have previously demonstrated that the reduced form of vitamin C (l-ascorbic acid, AA) is able to induce the production of both steroid and peptide hormones in human choriocarcinoma cells. Here, we attempted to investigate the role and underlying mechanism of the oxidized form of vitamin C, dehydroascorbic acid (DHA...

journal_title：Molecular human reproduction

authors： Wang Y,Tang C,Wu M,Pan Y,Ruan H,Chen L,Yao H,Zhu H,Wu X

更新日期：2014-08-01 00:00:00

abstract：:In the uterus, epithelial cell proliferation changes during the estrous cycle and pregnancy. Uncontrolled epithelial cell proliferation results in implantation failure and/or cancer development. Transforming growth factor-β (TGF-β) signaling is a fundamental regulator of diverse biological processes and is indispensab...

journal_title：Molecular human reproduction

authors： Gao Y,Li S,Li Q

更新日期：2014-08-01 00:00:00

abstract：:This study assessed the participation of amphiregulin (AREG) and bone morphogenetic protein 15 (BMP15) during maturation of bovine cumulus-oocyte complexes (COCs) on cumulus cell function and their impact on subsequent embryo development. AREG treatment of COCs enhanced blastocyst formation and quality only when in th...

journal_title：Molecular human reproduction

authors： Sugimura S,Ritter LJ,Sutton-McDowall ML,Mottershead DG,Thompson JG,Gilchrist RB

更新日期：2014-06-01 00:00:00

abstract：:Binder of SPerm (BSP) proteins are a family of proteins expressed exclusively in the male reproductive tract (seminal vesicles or epididymis) of several mammalian species. They are known to promote capacitation, a sperm maturation step essential for fertilization. Our recent studies have shown that in human, the Binde...

journal_title：Molecular human reproduction

authors： Plante G,Thérien I,Lachance C,Leclerc P,Fan J,Manjunath P

更新日期：2014-05-01 00:00:00

abstract：:Interleukin (IL)-33, a newly described member of the IL-1 family, has been reported to facilitate primary tumor progression and metastatic dissemination. However, its biological function on decidual stromal cells (DSCs) remains unclear. In this study, we tested the hypothesis whether IL-33 promotes proliferation and i...

journal_title：Molecular human reproduction

authors： Hu WT,Li MQ,Liu W,Jin LP,Li DJ,Zhu XY

更新日期：2014-04-01 00:00:00

abstract：:In mature mammalian oocytes, cortical f-actin distribution is polarized, as evidenced by a prominent cap subtended by the metaphase II (MII) spindle. Formation of a polarized actin cap is a consequence of a complex actomyosin-driven contractile process that directs polar body extrusion. Human mature oocytes also displ...

journal_title：Molecular human reproduction

authors： Coticchio G,Guglielmo MC,Albertini DF,Dal Canto M,Mignini Renzini M,De Ponti E,Fadini R

更新日期：2014-03-01 00:00:00

abstract：:Morphological assessments are the main way in which fertility clinics select in vitro generated embryo(s) for transfer to the uterus. However, it is widely acknowledged that the microscopic appearance of an embryo is only weakly correlated with its viability. Furthermore, the extent to which morphology is affected by ...

journal_title：Molecular human reproduction

authors： Fragouli E,Alfarawati S,Spath K,Wells D

更新日期：2014-02-01 00:00:00

abstract：:The molecular mechanism of stress-associated reproductive dysfunction is complex and largely unknown. This study was designed to systematically analyze molecular effects of systemic in vivo blockade of α1-adrenergic receptors (α1-ADRs) on stress-induced disturbance of cAMP/cGMP signaling in testosterone-producing Leyd...

journal_title：Molecular human reproduction

authors： Stojkov NJ,Baburski AZ,Bjelic MM,Sokanovic SJ,Mihajlovic AI,Drljaca DM,Janjic MM,Kostic TS,Andric SA

更新日期：2014-01-01 00:00:00

abstract：:In humans, the most common chromosomal abnormality is aneuploidy. Because the majority of aneuploid conceptuses die during the early stages of embryonic development, an accurate estimate of the frequency of aneuploidy at conception can only be assessed by directly studying the gametes. The vast majority of aneuploidie...

journal_title：Molecular human reproduction

authors： Templado C,Uroz L,Estop A

更新日期：2013-10-01 00:00:00

abstract：:Development of uterine glands (adenogenesis) in mammals typically begins during the early post-natal period and involves budding of nascent glands from the luminal epithelium and extensive cell proliferation in these structures as they grow into the surrounding stroma, elongate and mature. Uterine glands are essential...

journal_title：Molecular human reproduction

authors： Cooke PS,Spencer TE,Bartol FF,Hayashi K

更新日期：2013-09-01 00:00:00

abstract：:Oligozoospermia (low sperm count) is a common semen deficiency. However, to date, few genetic defects have been identified to cause this condition. Moreover, even fewer molecular genetic diagnostic tests are available for patients with oligozoospermia in the andrology clinic. Based on animal and gene expression studie...

journal_title：Molecular human reproduction

authors： Yatsenko AN,Georgiadis AP,Murthy LJ,Lamb DJ,Matzuk MM

更新日期：2013-06-01 00:00:00

abstract：:During early gestation, a considerable increase in different leukocyte subsets can be observed in the decidualized endometrium concomitantly to the invasion of cytotrophoblast cells (CTB). To date, it is still in question which factors induce this accumulation of immune cells and whether it is evoked by an in situ pro...

journal_title：Molecular human reproduction

authors： Segerer SE,Martignoni F,Bogdan A,Müller N,Kapp M,Dietl J,Rieger L,Kämmerer U

更新日期：2013-06-01 00:00:00

abstract：:The non-classical major histocompatibility complex molecule, human leukocyte antigen (HLA)-G, is thought to contribute to maternal immune tolerance and successful placentation during pregnancy. Genetic polymorphisms in HLA-G are known to influence expression levels as well as the relative expression of individual prot...

journal_title：Molecular human reproduction

authors： Loisel DA,Billstrand C,Murray K,Patterson K,Chaiworapongsa T,Romero R,Ober C

更新日期：2013-03-01 00:00:00

abstract：:Within the context of an oocyte in vitro maturation (IVM) program for reproductive treatment, oocyte cumulus complexes (COCs) derived from follicles <6 mm in patients with PCOS were matured in vitro. Key transcripts related to meiotic maturation (FSHR, LHCGR, EGFR, PGR) and oocyte competence (AREG, ADAMTS, HAS2, PTGS2...

journal_title：Molecular human reproduction

authors： Guzman L,Adriaenssens T,Ortega-Hrepich C,Albuz FK,Mateizel I,Devroey P,De Vos M,Smitz J

更新日期：2013-01-01 00:00:00

abstract：:Indoleamine 2,3-dioxygenase-1 (IDO1) is an intracellular enzyme that catalyses essential amino acid tryptophan along the kynurenine pathway. The aim of this study was to determine the impact of IDO1 expression on the biological characteristic of the endometrial stromal cells (ESCs). IDO1, cyclooxygenase-2 (COX-2) and ...

journal_title：Molecular human reproduction

authors： Mei J,Jin LP,Ding D,Li MQ,Li DJ,Zhu XY

更新日期：2012-10-01 00:00:00

abstract：:The pool of primordial follicles in humans is laid down during embryonic development and follicles can remain dormant for prolonged intervals, often decades, until individual follicles resume growth. The mechanisms that induce growth and maturation of primordial follicles are poorly understood but follicles once activ...

journal_title：Molecular human reproduction

authors： Markholt S,Grøndahl ML,Ernst EH,Andersen CY,Ernst E,Lykke-Hartmann K

更新日期：2012-02-01 00:00:00

abstract：:After meiosis, during the final stages of spermatogenesis, the haploid male genome undergoes major structural changes, resulting in a shift from a nucleosome-based genome organization to the sperm-specific, highly compacted nucleoprotamine structure. Recent data support the idea that region-specific programming of the...

journal_title：Molecular human reproduction

authors： Govin J,Gaucher J,Ferro M,Debernardi A,Garin J,Khochbin S,Rousseaux S

更新日期：2012-01-01 00:00:00

abstract：:Endometriosis, the presence of ectopic endometrial tissue outside the uterine cavity, is a common disease affecting women during their reproductive years. The aim of this study was to identify the molecular mechanism of transcriptional regulation of inflammatory cyclooxygenase-2 (COX-2) gene during endometriosis by hy...

journal_title：Molecular human reproduction

authors： Kim KH,Kim HY,Kim HH,Lee KS,Cheong J

更新日期：2011-11-01 00:00:00

abstract：:We investigated the expression of methyl transferase G9a and methylated histone H3-K9 in fresh human decidual/endometrial tissue of 12 normal early pregnancies and 15 unexplained recurrent spontaneous abortions (URSA). The samples were obtained through dilatation and curettage and collected as per strict inclusion-exc...

journal_title：Molecular human reproduction

authors： Fatima N,Ahmed SH,Salhan S,Rehman SM,Kaur J,Owais M,Chauhan SS

更新日期：2011-11-01 00:00:00

abstract：:Regulation of germ-cell-specific transcription is essential for the differentiation and other physiological processes of germ cells. Germ-cell-specific transcription factors, Sohlh2 and Figla, play key roles in gametogenesis. To elucidate whether an epigenetic mechanism is involved in the controlled expression of Sohl...

journal_title：Molecular human reproduction

authors： Pan B,Chao H,Chen B,Zhang L,Li L,Sun X,Shen W

更新日期：2011-09-01 00:00:00